Magnetic resonance evaluation of human mesenchymal stem cells in corpus cavernosa of rats and rabbits / 亚洲男科学杂志(英文版)

<p><b>AIM</b>To investigate whether the biological process of superparamagnetic iron oxide (SPIO)-labeled human mesenchymal stem cells (hMSCs) may be monitored non-invasively by using in vivo magnetic resonance (MR) imaging with conventional 1.5-T system examinations in corpus cavernosa of rats and rabbits.</p><p><b>METHODS</b>The labeling efficiency and viability of SPIO-labeled hMSCs were examined with Prussian blue and Tripan blue, respectively. After SPIO-labeled hMSCs were transplanted to the corpus cavernosa of rats and rabbits, serial T2-weighted MR images were taken and histological examinations were carried out over a 4-week period.</p><p><b>RESULTS</b>hMSCs loaded with SPIO compared to unlabeled cells had a similar viability. For SPIO-labeled hMSCs more than 1 X 10 (5) concentration in vitro, MR images showed a decrease in signal intensity. MR signal intensity at the areas of SPIO-labeled hMSCs in the rat and rabbit corpus cavernosa decreased and was confined locally. After injection of SPIO-labeled hMSCs into the corpus cavernosum, MR imaging demonstrated that hMSCs could be seen for at least 12 weeks after injection. The presence of iron was confirmed with Prussian blue staining in histological sections.</p><p><b>CONCLUSION</b>SPIO-labeled hMSCs in corpus cavernosa of rats and rabbits can be evaluated non-invasively by molecular MR imaging. Our findings suggest that MR imaging has the ability to test the long-term therapeutic potential of hMSCs in animals in the setting of erectile dysfunction.</p>

Molecular MRI Images of the Transplanted Human Mesenchymal Stem Cells in the Liver, Kidney, Bladder and Penile Cavernosum of Rats / 대한비뇨기과학회지

PURPOSE: Molecular magnetic resonance (MR) imaging techniques using superparamagnetic iron oxide nanocrystals (SPIO) have been developed for noninvasively monitoring stem cells. This study was performed to investigate if the presence of transplanted human mesenchymal stem cells in the liver, kidney, bladder and penile cavernosum can be evaluated noninvasively with using molecular MR imaging. MATERIALS AND METHODS: SPIO (Feridex; AMI, Cambridge, USA) were transferred to the human mesenchymal stem cells (hMSCs) using GenePORTER. The labeling viability, efficiency and differentiation of the SPIO transferred hMSCs were examined with Tripan blue, Von Kossa, alkaline phosphatase, toluidine blue, oil red O and Prussian blue staining. The SPIO labelled hMSCs were transplanted to the liver, kidney, bladder and penile cavernosum of rats, and the MR images were examined in vitro or in vivo using 1.5 T MR. RESULTS: The viability and efficiency of the SPIO transferred hMSCs were good. Osteogenic, chondrogenic or adipogenic differentiation from the SPIO transferred hMSCs was observed. A decrease of the MR signal intensity of the SPIO transferred hMSCs with using GenePORTER was found in vitro. A decrease of the MR signal intensity was found at concentrations that were more than 1x10(5) hMSCs in vitro. The MR signal intensity at the areas of the SPIO transferred hMSCs decreased in the liver, kidney, bladder and penile cavernosum. The intracellular SPIOs were confirmed in the SPIO labelled hMSCs that were transplanted in the liver, kidney, bladder and penile cavernosum with Prussian blue staining. CONCLUSIONS: The SPIO labelled hMSCs in the liver, kidney, bladder and penis can be evaluated noninvasively by using molecular MRI.

Histologic Monitoring of the Transplanted Superparamagnetic Iron Oxide Labelled Human Mesenchymal Stem Cells in the Rat Bladder

PURPOSE: Molecular magnetic resonance(MR) imaging technique using the superparamagnetic iron oxide nanocrystals(SPIO) are developed for the monitoring the stem cells noninvasively. This study was performed to monitor the transplanted SPIO labelled human mesenchymal stem cells(hMSCs) with the molecular MR imaging and histologically in the rat bladder. MATERIALS AND METHODS: SPIO(Feridex; AMI, Cambridge, MA, USA) were labelled to hMSCs using GenePORTER. The labeling viability, efficiency of the SPIO labelled hMSCs were examined with tripan blue, prussian blue staining. SPIO labelled hMSCs are transplanted to the bladder in rats and MR images of them were examined using 1.5 T MR. Histologic examination of the SPIO labelled hMSCs transplanted bladder was performed with H&E and prussian blue staining. RESULTS: The viability, efficiency of the SPIO labelled hMSCs were good. MR signal intensity at the areas of the SPIO labelled hMSCs in the bladder decreased after transplantation of 1x10(6) SPIO labelled hMSCs and were confined locally. Intracellular SPIO were confirmed in the hMSCs transplanted bladder up to 5 weeks. CONCLUSION: The SPIO labelled hMSCs in the bladder can be monitored using the molecular MR imaging and SPIO labelled hMSCs were found in the transplanted bladder.

Monitoring Transplanted Human Mesenchymal Stem Cells in the Rabbit Bladder Using Molecular MRI / 대한남성과학회지

PURPOSE: Monitoring the biological changes in transplanted stem cells is important. Molecular magnetic resonance (MR) imaging using superparamagnetic iron oxide nanocrystals(SPIO) has been developed for noninvasive monitoring of stem cells. This study was performed to investigate whether the biological status of transplanted human mesenchymal stem cells(hMSCs) in the rabbit penis can be monitored noninvasively using molecular MR imaging. MATERIALS AND METHODS: SPIO(Feridex; AMI, Cambridge, MA, USA) were transferred into hMSCs using GenePORTER. The labeling efficiency, viability, and differentiation of the SPIO-containing hMSCs were examined with Trypan blue, Von Kossa, alkaline phosphatase, toluidine, blueoil red O, and Prussian blue staining. SPIO-labeled hMSCs were transplanted into the rabbit penis, and MR images of them were examined in vitro or in vivo using 1.5 T MR. Histologic examination was performed. RESULTS: The viability and efficiency of the SPIO-transferred hMSCs were good. Osteogenic, chondrogenic and adipogenic differentiation from SPIO-containing hMSCs were found. A decrease in MR signal intensity in the SPIO-containing hMSCs was found in vitro. MR signal intensity in the areas of the SPIO-transferred hMSEc in the rabbit penis decreased and were confined locally. Intracellular SPIO were confirmed in the hMSCs transferred into penis. CONCLUSIONS: SPIO-labeled hMSCs in the rabbit penis can be evaluated noninvasively using molecular MR imaging.

Monitoring Transplanted Human Mesenchymal Stem Cells in the Rabbit Bladder Using Molecular MRI / 대한남성과학회지

PURPOSE: Monitoring the biological changes in transplanted stem cells is important. Molecular magnetic resonance (MR) imaging using superparamagnetic iron oxide nanocrystals(SPIO) has been developed for noninvasive monitoring of stem cells. This study was performed to investigate whether the biological status of transplanted human mesenchymal stem cells(hMSCs) in the rabbit penis can be monitored noninvasively using molecular MR imaging. MATERIALS AND METHODS: SPIO(Feridex; AMI, Cambridge, MA, USA) were transferred into hMSCs using GenePORTER. The labeling efficiency, viability, and differentiation of the SPIO-containing hMSCs were examined with Trypan blue, Von Kossa, alkaline phosphatase, toluidine, blueoil red O, and Prussian blue staining. SPIO-labeled hMSCs were transplanted into the rabbit penis, and MR images of them were examined in vitro or in vivo using 1.5 T MR. Histologic examination was performed. RESULTS: The viability and efficiency of the SPIO-transferred hMSCs were good. Osteogenic, chondrogenic and adipogenic differentiation from SPIO-containing hMSCs were found. A decrease in MR signal intensity in the SPIO-containing hMSCs was found in vitro. MR signal intensity in the areas of the SPIO-transferred hMSEc in the rabbit penis decreased and were confined locally. Intracellular SPIO were confirmed in the hMSCs transferred into penis. CONCLUSIONS: SPIO-labeled hMSCs in the rabbit penis can be evaluated noninvasively using molecular MR imaging.